- June 24, 2020
3-Phosphate Dehydrogenase (GAPDH; EC 22.214.171.124) catalyzes the conversion of Glyceraldehyde-
3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism, and nuclear translocation. GAPDH is a stable metabolic enzyme and is constitutively expressed. Therefore, it is considered as a housekeeping gene. Deregulation of GAPDH activity is associated with cell proliferation and carcinogenesis. Accurate detection of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology. BioVision's Human GAPDH Activity Assay Kit II provides a simple and sensitive method for monitoring GAPDH activity in various sample types. In this assay, GAPDH catalyzes GAP into BPG and an intermediate, which then reacts with the developer to form a colored product that is measured at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 10 mU/mL in a variety of samples.
Figures: a). NADH Standard Curve. b). GAPDH activity in HeLa and Jurkat cell lysates. c). Specific GAPDH activity was calculated in cell lysates prepared from Jurkat (0.8 µg), HeLa (0.4 µg), and HEK293 cell lysates (0.4 µg). Assays were performed following the kit protocol.
For more information on this Assay Kit, visit: https://www.biovision.com/