- Feb. 9, 2020
-- Molecular Depot accounces the availibility of a new beta-galacctosidase substrate: CPRG (Chlorophenol red-β-D-galactopyranoside)
Chlorophenol red-β-D-galactopyranoside (CPRG) is an excellent beta-galactosidase substrate that surpasses o-nitrophenyl-
beta-D-galactopyranoside (ONPG). CPRG is very soluble in water and its chromogenic product (chlorophenol red) has an extinction coefficient that is 21-fold higher than that of ONPG (o-nitrophenol)
. As a result, CPRG can be used in highly sensitive beta-galactosidase-
based assays and detection methods. Absorbance of chlorophenol red can be measured at 570-595 nm.
CPRG is particularly useful in Beta-galactosidase Alpha-Complementation, a biochemical phenomenon first documented by Agnes Ullmann, while working in the lab of François Jacob and Jacques Monod. By means of molecular cloning, the native E. coli
β-galactosidase enzyme can be split in two inactive fragments of different sizes. The smaller fragment, known as the alpha-peptide or enzyme donor, is about 100 amino residues in length and is inactive on its own (incapable of hydrolyzing a β-galactosidase substrate). The larger fragment, known as the omega fragment or enzyme acceptor, is about 900 amino residues in length and is also inactive on its own. Upon mixing the enzyme donor with the enzyme acceptor, the β-galactosidase enzyme is reconstituted and is now capable of hydrolyzing colorimetric substrates such as ONPG and CPRG.
For more information about Molecular Depot's CPRG, please visit:https://moleculardepot.com/product/cprg-chlorophenol-red-...