- May 24, 2019
-- Testing antibiotic susceptibility in bacterial isolates enables the detection of drug resistance in common bacterial pathogens. This ensures susceptibility to selected pharmaceutical interventions for specific pathogens classified as gram negative rods. The broth microdilution, a well-known method in microbiology is flexible and provides quantitative and semi-quantitative results to monitor resistance amongst bacterial pathogens. It is called microdilution because it involves the use of small volumes of broth dispensed in sterile, plastic microdilution trays (~0.1 ml of broth).
BioVision's Minimal Inhibitory Concentration (MIC) kit, testing for Gram Negative Rods (GNR) is a broth microdilution assay that is simple yet sensitive for the quantification of any antimicrobial agent against a given bacterial isolate. The antibiotic efficacy is quantified using a Water Soluble Tetrazolium salt (WST) which changes color reflecting the viability of the bacteria as a response to antibiotic treatment. The test is performed in a microplate with bacteria-inoculated-
Luria Bertani (LB) broth and serial dilutions of antimicrobial agents can be added. Our kit includes four antibiotics often prescribed for infections caused by gram negative rods. They are Ampicillin Sodium, Chloramphenicol, Enrofloxacin, and Gentamicin Sulfate. Vancomycin Hydrochloride, an antibiotic effective in the treatment of gram positive cocci, is included as a negative control. The Antibiotic Susceptibility Limits of these antibiotics have been evaluated and correlatedwith published standards (Andrews, 2001) for MICs.Figure:
Vancomycin (MIC 0.1 mg/ml, 8hr). Measurement after addition of the detection system (WST/ECS) was performed following kit K195 protocol for 2 hr at 37ºC.
For more information on this kit, visit: https://www.biovision.com/minimal-inhibitory-concentration-kit-22385.html