MONMOUTH JUNCTION, N.J.
- Dec. 15, 2018
-- Biotech Support Group reports on a recent research article describing the simplicity and efficiency of their lipid clearance sample preparation technology for studying cell response factors associated with cells of the central nervous system. The citation is:
Sprenkle, Neil T., et al. "Endoplasmic reticulum stress is transmissible in vitro between cells of the central nervous system (https://onlinelibrary.wiley.com/
jnc.14642)." Journal of Neurochemistry
Improper protein folding and trafficking are common pathological events in neurodegenerative diseases that result in the toxic accumulation of misfolded proteins within the lumen of the endoplasmic reticulum (ER). Yet the cell‐extrinsic role of sustained unfolded protein response activation under physiological and pathological states in the central nervous system (CNS) remains to be elucidated. The authors studied the characteristics of a mediator secreted by ER stressed astrocytes and neurons. To determine if the mediator had lipid characteristics, the article states "…100 μl of Cleanascite slurry was added to 1 ml of conditioned medium and incubated at RT with end-over-end mixing for 1 h followed by centrifugation."
The authors provided evidence that depletion of lipids from astrocyte conditioned media using Cleanascite abrogated transmission of ER stress. Such evidence helped the authors conclude that ER stressed astrocytes and neurons secrete a molecule(s) with lipid characteristics which regulates both inflammatory and ER stress responses in other astrocytes, neurons, and microglia in vitro
. These findings provide insight into the cell-nonautonomous influence of ER stress on cells of the central nervous system.
"I am pleased that using Cleanascite™
helped to identify the characteristic feature of an unknown mediator of cell response. This demonstrates the advantage of using an aqueous suspension product with no harmful solvents, so one can challenge cells for responsiveness. This ultimately helps researchers decide whether any factor is or is not consistent with a biomolecule having lipid characteristics. We highlight many references in this area on our recently published whitepaper."
states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
To download whitepaper entitled "Cleanascite™ - Lipid Removal and Cell Response Applications
For more information visit: Cleanascite™
Lipid Removal Reagent and Clarification, athttp://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htmAbout Biotech Support Group LLC
Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For this market, key products include: AlbuVoid™ and AlbuSorb™ for albumin depletion, Cleanascite™
for lipid adsorption, HemogloBind™
and HemoVoid™ for hemoglobin removal, and NuGel™ for functional proteomics. From these innovations, the company has acquired knowledgebase and biomarker intellectual property assets that support discoveries of protein markers from blood, with special emphasis on early detection and personalized medical decisions for cancer patients. For more information, go to http://www.biotechsupportgroup.com
Matthew Kuruc 732-274-2866, firstname.lastname@example.org