MONMOUTH JUNCTION, N.J.
- Nov. 17, 2018
-- Biotech Support Group reports on a recent research article describing the simplicity and efficiency of their lipid clearance sample preparation technology for studying factors associated with interrupted glucagon signaling. The citation is:
Dean, E. Danielle, et al. "Interrupted glucagon signaling reveals hepatic α cell axis and role for L-glutamine in α cell proliferation (https://www.sciencedirect.com/
." Cell metabolism
25.6 (2017): 1362-1373.
Decreasing glucagon action lowers blood glucose and may be useful therapeutically for diabetes. However, interrupted glucagon signaling leads to α cell proliferation. In this article, the authors wanted to determine which factors affected α cell proliferation. The article states "For lipid removal, whole mouse serum was treated with Cleanascite™
reagent (Biotech Support Group, Monmouth Junction, NJ) prior to islet culture at a 1:1 ratio according to the vendor's protocol. Lipid removal was validated by HPLC to remove 99% of all phopsholipids, cholesterols, and triglycerides…
.". In testing whether lipids could stimulate α cell proliferation, we found that serum activity was retained after the removal of >99% of triglycerides, cholesterols, and phospholipids. The authors conclude that amino acids, especially L-glutamine, regulate α cell proliferation and mass via mTOR-dependent nutrient sensing.
"This is another application whereby using Cleanascite™
demonstrates the advantage of using an aqueous suspension product with no harmful solvents, to deplete lipid associated factors that may influence cell response investigations."
states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
For more information visit: Cleanascite™
Lipid Removal Reagent and Clarification, athttp://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htmAbout Biotech Support Group LLC
Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For this market, key products include: AlbuVoid™ and AlbuSorb™ for albumin depletion, Cleanascite™
for lipid adsorption, HemogloBind™
and HemoVoid™ for hemoglobin removal, and NuGel™ for functional proteomics. From these innovations, the company has acquired knowledgebase and biomarker intellectual property assets that support discoveries of protein markers from blood, with special emphasis on early detection and personalized medical decisions for cancer patients. For more information, go to http://www.biotechsupportgroup.comContact:
Matthew Kuruc 732-274-2866