MILPITAS, Calif. -
July 18, 2018 -
PRLog -- Methylglyoxal (MG, 2-oxopropanal, pyruvic aldehyde), a by-product of glycolysis, is a highly toxic, reactive dicarbonyl compound. It causes non-enzymatic glycation of proteins which yields irreversible advanced glycation end products (AGEs), leading to cross-linking or degradation of proteins. MG is associated with several pathologies including diabetes, aging and neurodegenerative diseases. Furthermore, recent studies have suggested that MG may induce carbonyl stress-type schizophrenia. BioVision's PicoProbe
TM Methylglyoxal Assay Kit (Fluorometric)
can detect MG using an enzyme-coupled reaction which reduces the fluorogenic probe. The reduced fluorophore produces a stable signal (Ex/Em= 535/587nm), which is directly proportional to the amount of MG in samples. The assay is simple, reproducible and can specifically detect as low as 6 pmol of MG in a 100 µl reaction.
FIGURE. Measurement of Methylglyoxal (K461-100) in Mouse Liver (4 mg tissue), Rat Brain (12 mg tissue) and Human Cerebrospinal Fluid (CSF, 2 µl). All assays were performed following kit protocol.
For complete information on this assay kit, visit:
https://www.biovision.com/picoprobetm-methylglyoxal-assay...