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Research Article Cites NuGel™ PBA for Proteome Analysis of Saliva
The citation is:
Gutiérrez, Ana, et al. "Influence of different sample preparation strategies on the proteomic identification of stress biomarkers in porcine saliva." BMC Veterinary Research 13.1 (2017): 375. https://bmcvetres.biomedcentral.com/
The authors describe the influence of two different sample pre-treatments on the detection of stress biomarkers in saliva. Both the enrichment of glycoproteins by immobilized boronic acid (PBA) and sub-proteome enrichment by dynamic range compression were considered in this study. For this purpose, saliva samples collected before and after the application of an acute stress model in pigs were evaluated. Two-dimensional gel electrophoresis (2-DE) followed by MALDI-TOF/TOF mass spectrometry (MS) was used as the proteomic reporting technique. The article states "1mg of total protein from each salivary pool was lyophilized and subjected to PBA treatment. A commercial glycoprotein enrichment kit based on PBA matrix was used (NuGel™ Glycoprotein enrichment PBA kit, Biotech Support Group) according to the manufacturer's instructions."
"This reference shows the versatility of NuGel™ PBA to efficiently enrich glycoproteins from a readily accessible biofluid, with a selectivity profile suitable for proteome analysis and biomarker discovery using conventional proteomic analyses." states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
For more information on glycoprotein enrichment products, visit:
ForNuGel™ Phenyl Boronic Acid:
ForNuGel™ Glycoprotein PBA Enrichment Kit:
About Biotech Support Group LLC
Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For this market, key products include: AlbuVoid™ and AlbuSorb™ for albumin depletion, Cleanascite™
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