Research Article Cites NuGel™ PBA for Proteome Analysis of Saliva

MONMOUTH JUNCTION, N.J. - Dec. 14, 2017 - PRLog -- Biotech Support Group reports on a recent research article describing the simplicity and efficiency of their NuGel™ PBA glycoprotein enrichment sample preparation technology for the proteomic analysis of saliva.

The citation is:

Gutiérrez, Ana, et al. "Influence of different sample preparation strategies on the proteomic identification of stress biomarkers in porcine saliva." BMC Veterinary Research 13.1 (2017): 375. https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1296-9

The authors describe the influence of two different sample pre-treatments on the detection of stress biomarkers in saliva. Both the enrichment of glycoproteins by immobilized boronic acid (PBA) and sub-proteome enrichment by dynamic range compression were considered in this study. For this purpose, saliva samples collected before and after the application of an acute stress model in pigs were evaluated. Two-dimensional gel electrophoresis (2-DE) followed by MALDI-TOF/TOF mass spectrometry (MS) was used as the proteomic reporting technique. The article states "1mg of total protein from each salivary pool was lyophilized and subjected to PBA treatment. A commercial glycoprotein enrichment kit based on PBA matrix was used (NuGel™ Glycoprotein enrichment PBA kit, Biotech Support Group) according to the manufacturer's instructions." In brief, "the freeze-dried PBA saliva was dissolved in 250μL of binding buffer, added to 50mg of PBA matrix and incubated for 10min. The unbound fraction was discarded…the matrix was washed three times with 350μL of wash buffer, and the glycoprotein-enriched fraction was obtained by incubation of the matrix with 300μL of sorbitol elution buffer…". The authors concluded that the use of different procedures allowed the detection of different potential stress biomarkers. From a practical point of view, the use of saliva without treatment as well as the enrichment of glycoproteins with NuGel™ PBA were preferred.

"This reference shows the versatility of NuGel™ PBA to efficiently enrich glycoproteins from a readily accessible biofluid, with a selectivity profile suitable for proteome analysis and biomarker discovery using conventional proteomic analyses." states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

For more information on glycoprotein enrichment products, visit:

ForNuGel™ Phenyl Boronic Acid:

http://www.biotechsupportgroup.com/NuGel-Phenyl-Boronic-Acid-p/npba.htm

ForNuGel™ Glycoprotein PBA Enrichment Kit:

http://www.biotechsupportgroup.com/NuGel-Glycoprotein-Enrichment-PBA-Kit-p/ngpba.htm

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For this market, key products include: AlbuVoid™ and AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal, and NuGel™ for functional proteomics. From these innovations, the company has acquired knowledgebase and biomarker intellectual property assets that support discoveries of protein markers from blood, with special emphasis on early detection and personalized medical decisions for cancer patients. For more information, go to http://www.biotechsupportgroup.com (http://www.biotechsupportgroup.com/biotechsupportgroup.com).

Contact: Matthew Kuruc 732-274-2866 mkuruc@biotechsupportgroup.com (mailto:sales@biotechsupportgroup.com)