The most sensitive Methyltransferase Activity Assay Kits

MILPITAS, Calif. - Sept. 23, 2016 - PRLog -- Methyltransferases are a diverse group of enzymes that methylate proteins, DNA, and small molecule targets. Accordingly, methyl transferase activity plays a critical role in modulating gene transcription, protein interactions, and signaling networks. BioVision is excited to introduce two Methyltransferase Activity Assay Kits (Colorimetric & Fluorometric) that allow for kinetic evaluation ofmethyltransferase activity of purified enzymes and their inhibitors. Methyltransferase activity is detected by coupling the methyl transfer reaction to a multi-step enzymatic cascade. These kits are highly sensitive, ready-to-use and can detect Methyltransferase activities as low as 10 µU/ml of purified enzymes.
Key Features:
• Non-radioactive
• Specific, homogeneous assays
• Versatile: colorimetric and fluorometric formats
• Convenient: minimal sample preparation, fast protocols (1-2 hours)
• Cost-effective: 100 assays
• Adaptable: works with most of the commonly available instrumentations
Figure-A. Representative activity curve for human recombinant Nicotinamide N-Methyltransferase (NNMT) using K986-100 at 37°C. Figure-B. representative activity curve for human recombinant Protein Arginine Methyltransferase 1 (hPRMT1) with Histone H4 Substrate using K521-100 at 25°C.
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