Hands on Training Program in Real Time PCR (qPCR) Technique

ABU DHABI, UAE - July 25, 2016 - PRLog -- The Polymerase Chain Reaction (PCR) is a biochemical technology in molecular biology

used to amplify a single copy or a few copies of a piece of DNA across several orders of

magnitude, generating thousands to millions of copies of a particular DNA sequence. PCR

concept is the first step in all DNA and RNA based research including RFLP, TRFLP, AFLP,

Real Time PCR, DNA sequencing, Microarray, Next Generation Sequencing and so on.

People who are working in diagnostic laboratories like medical, food and environmental

may be trained or educated based on the traditional diagnosis methods. They are using

microbiological plating technique for bacterial disease diagnosis and for results they have

to wait for at least 48 hours. For diagnosis of viral and parasitic diseases, mostly

symptoms and conventional PCR methods, which will give only the qualitative results

and not about the quantity of that virus or parasites (disease severity).

Most of the diagnosis laboratories have Real time PCR machine. Therefore, the

laboratory technician and biotechnology students need to have good hands on

experience in Real Time PCR. By using this technique, diagnosis will be completed in 3-4

hours and analysed qualitatively and quantitatively. Moreover, we can detect more than

two causative agents (>2 bacteria) in a medical or environmental samples. This training

will provide you complete good laboratory practices (GLP) in molecular biology

laboratory and trouble shooting in PCR and Real Time PCR.

Training Program Objectives

1. PCR technique for refreshment

 Extraction of DNA from human blood

 PCR for human gene (GAPDH) from extracted DNA

 Agarose gel electrophoresis of DNA and PCR product

2. Quantification of DNA by Real Time PCR

 Quantification using GAPDH or 5S primers

 Real Time reaction set up

 Quantitative analysis of results using software

3. Real Time PCR for Hepatitis C Virus (HCV) detection from Plasma or

Serum samples

 Total RNA extraction from plasma or serum

 Real Time reaction set up

 Quantitative analysis of results using software

Information

 Two day training program

 Eligibility- UG/PG/PhD students, faculty, scientists as well as people working in

industry in the field of Biotechnology, Bioinformatics, Life Sciences, Medical

Sciences, Pharmaceutical Sciences, Chemical Sciences and related subject areas.

 Totally 12 participants can be accommodated and will be given hands on training.

 Registration

 AED. 600/- as program fee (includes registration fee and training materials) for

one participant.

 Registration is based on "First come first serve".

Date – August 24th and 25th 2016 (two days)

Venue - College of Biotechnology, University of Modern Sciences, Al Twar-3, Dubai, UAE

more details: sales@accuvisbio | Visit www.accuvisbio.com for details