Direct Method to Measure Cell Mediated Cytotoxicity

No Need to Rely on Indirect Indicators (LDH/ATP) Any longer!
 
 
Cell-Mediated Cytotoxicity Fluorometric Assay (7-AAD/CFSE)
Cell-Mediated Cytotoxicity Fluorometric Assay (7-AAD/CFSE)
MILPITAS, Calif. - Aug. 1, 2014 - PRLog -- Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by the immune system. Activation of immune system leads to removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. BioVision’s Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE) (K315-100) includes carboxyfluorosuccinimide ester (CFSE), a green fluorescent probe that labels live cells and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that labels late apoptotic and necrotic cells.

Key Features and Benefits of the Kit:
- Non-radioactive and highly sensitive
- Direct assay: Does not require cell lysis
- Flow Cytometry-based quantitation: robust and multi-parametric data analysis
- Measures cytotoxicity in response to drugs, toxins, antibodies and immune effector on target cells

For more information, please visit
http://www.biovision.com/cell-mediated-cytotoxicity-fluor...
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