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Follow on Google News | RNA Clean-Up and Concentration Kit is now with AccuVis Bio, Abu Dhabi, UAEAccuvis’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols...
By: AccuVis Bio Purification is based on spin column chromatography using Accuvis’s proprietary resin as the separation matrix. Briefly, the RNA samples or reactions containing RNA are mixed with Binding Solution, ethanol is added and the RNA is bound to Accuvis's column (BIND). Under these conditions only the RNA will bind to Accuvis's resin while most of the contaminating proteins and nucleotides are removed in the flowthrough. The bound RNA is then washed to remove any remaining impurities (WASH). Lastly, the purified total RNA is eluted into 20 - 50 µL of the provided Elution Buffer or water (ELUTE). Please see procedure flowchart to the right. The purified RNA is of the highest integrity, and can be used in a number of downstream applications. Accuvis’s RNA Clean-Up and Concentration Kit purifies RNA with minimal amounts of DNA contamination. An optional protocol is provided for maximum removal of residual DNA that may affect sensitive downstream applications such quantitative PCR. This kit is also available in a 96-well format for high-throughput RNA clean-up and concentration. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation. Efficient clean-up from various enzymatic reactions - RNA clean-up from upstream enzymatic applications such as labeling, DNase treatment and in vitro transcription. Remove proteins, RNases, DNases, nucleotides, etc. Clean-up and concentration of RNA isolated by different methods - RNA that has been isolated using various methods, including phenol-chloroform extractions or alcohol precipitations, can be processed with this kit Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes, while the 96-well plates can be processed in 30 minutes No phenol:chloroform extractions - Accuvis’s RNA Clean-Up and Concentration Kit purifies RNA without the use of harmful chemicals such as phenol or chloroform. Isolate all small RNA molecules - All small RNA species can be isolated including microRNA, siRNA, tRNA, and 5S rRNA Recovered RNA is suitable for downstream applications - Purified RNA can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, and expression array analysis for a complete review on our products, please visit: http://www.accuvisbio.com/ End
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