Monoclonal Antibody Sample Clarification & Chromatographic Column Life Extension Via Cleanascite™

Cleanascite™ is an ideal sample clarification and purification reagent for physicochemical fractionation antibody purification, class-specific affinity purification of antibodies, antigen-specific affinity purification of antibodies.
Cleanascite HC2pr
Cleanascite HC2pr
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* Monoclonal Antibody Purification
* Proteomics
* Drug Discovery

* Pharmaceuticals
* Biotechnology

* Monmouth Junction - New Jersey - US

* Features

Sept. 6, 2012 - PRLog -- MONMOUTH JUNCTION, NJ.– Cleanascite™ from Biotech Support Group selectively removes lipids, cell debris, lipoproteins, floating fats, impurities from cohn paste, transgenic milk, egg yolk and biological samples for pretreatment of samples prior to purification. Cleanascite™ is an ideal sample clarification and purification reagent for physicochemical fractionation antibody purification, class-specific affinity purification of antibodies, antigen-specific affinity purification of antibodies. It is compatible with large scale antibody purification protocols. During the development process of anti-immunoglobulin E (IgE) antibodies, Cleanascite™ is required because it is a solid phase, non-ionic adsorbent and this specialty technology makes it great for initial purification. It complements other purification biotechniques such as gel filtration, dialysis, ammonium sulfate precipitation or ion exchange chromatography or hydrophobic interaction. Moreover, Cleanascite™ is the right choice for experiments in which affinity chromatography cannot be implemented or if more purity is needed. For example, protein G and protein A are not bound to IgE therefore Cleanascite™ is the right choice.

After recovery and harvest of antibodies, Cleanascite™ removes impurities during the purification process of cell debris resulting in samples which are clarified from lipids, cell debris, lipoproteins, floating fats, impurities and it extends the life of membrane and chromatographic columns. The reagent is a solid-phase, non-ionic adsorbent supplied as a suspension in saline, ready for use. Simply add, centrifuge and/or filter. The clarified supernatant is ready for subsequent downstream processing or analysis.

Characteristics Of Cleanascite™

Does not bind to DNA, RNA, enzymes and proteins
Leaves glycoproteins, antibodies, nucleic acids, hemoglobin, proteoglycans, nucleic acids, serum components(such as hormones, nutrients, globulins, clotting factors, transport proteins) alone
Extends the life of membrane and chromatographic columns.
Enrichment of delipidated tissue samples
Ideal for delipidation treatments for downstream processing of large-scale therapeutic proteins, enzymes and monclonal antibodies.

For more information, visit:
Cleanascite™ Lipid Removal Reagent and Clarification

About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include:  AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, and ProCipitate™ & ProPrep™ for nucleic acid isolation. Currently, Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a proteomics platform used jfmnr in functional profiling for proteomic analysis and a separations method for generating sub-proteomes used in biomarker and functional proteomic prospecting. For more information, go to:

Dr.Swapan Roy
Biotech Support Group
1 Deer Park Drive, Suite M,
Monmouth Junction, NJ 08852, USA              

Cleanascite™ References

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Guerrier L, Claverol S, Finzi L et al. Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins. Journal of Chromatography.2007;1176(1-2):192-205
Chen Bo, Zheng Jian-wei, Wang Jian-ming, et al. Establishment and preliminary analysis of a 2-D human biliary map Chinese Journal of Hepatobiliary Surgery.2007
Chen B, Dong JQ, Chen YJ et al Two-dimensional electrophoresis for comparative proteomic analysis of human bile. Hepatobiliary & pancreatic diseases international.2007 Aug;6(4):402-6
Guerrier L, Claverol S, Finzi L et al Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins.Journal of Chromatography A.2007;1176(1-2):192-205
Kristiansen TZ, Bunkenborg J, Gronborg M et al A Proteomic Analysis of Human Bile Molecular and Cellular Proteomics.2004;3:715-728

Organ Homogenates
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Lijowski M, Caruthers S, Hu G. High-Resolution SPECT-CT/MR Molecular Imaging of Angiogenesis in the Vx2 Model Investigative Radiology.2009;44(1): 15–22
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Suggested References:
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Guse A, Milton A, Schulze-Koops H, Muller B, Roth E, Simmer B. Purification and analytical characterization of an anti-CD4 monoclonal antibody for human therapy. J Chromatogr A. 1994;661:13–23
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Tags:Monoclonal Antibody Purification, Proteomics, Drug Discovery
Industry:Pharmaceuticals, Biotechnology
Location:Monmouth Junction - New Jersey - United States
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