Update on Glycomics - Achieve Safe, Easy, and Accurate O-Glycan Analysis with BlotGlyco® O-Glycan

GÖTTINGEN, Germany - Nov. 17, 2015 - PRLog -- Hydrazinolysis is a traditional method for O-glycan release from glycoproteins. However, this method requires anhydrous hydrazine, a highly toxic and hard to handle compound. Another problem is "peeling", which is an unwanted O-glycan degradation during the release process of conventional methods. Traditional hydrazinolysis, as well as some O-glycan release kits on the market, can not avoid peeling.

The BlotGlyco^® O-Glycan Sample Preparation Kit can release O-glycan under mild alkaline conditions, thereby eliminating this peeling reaction. The kit does not require harsh reagents such as hydrazine, hence researchers are able to obtain O-glycan samples easily and safely.

BlotGlyco^® O-Glycan Sample Preparation Kit is perfectly suited for O-glycan analysis of a broad range of biological samples such as purified glycoproteins and crude samples. Examples are unpurified glycoproteins, serum, urine, cell and tissue lysates, and plant extracts.

Applications

• O-Glycosylation analysis of biologics.
• For research, screening, quality control, production process development, and monitoring.
• O-Glycosylation analysis of biological samples such as mucins.

Features

• The kit facilitates O-glycan sample preparation from biological samples such as glycoproteins, mucins, etc.
• It consists of O-glycan release reagent and glycan purification/labeling beads.
• Eliminating time-consuming tedious purification steps.
• Only two days are required to obtain a purified, 2-AB labeled O-glycan sample from biological samples.
• Safer than conventional methods due to the usage of a mild ammonium salt for O-glycan release.
• Easy operations. No need for special devices.

MoBiTec GmbH, Germany, is certified agent for Sumitomo Bakelite Co., Ltd., in Europe.

About MoBiTec GmbH

MoBiTec GmbH (Goettingen, Germany) is a privately held company that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.

In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.