Dolomite creates unique microfluidics chip for creation of single cell libraries

Nov. 4, 2015 - PRLog -- Dolomite, a world leader in microfluidics innovation, has launched the Single Cell RNA-Seq Chip for simple, reliable creation of single cell libraries. This new chip allows researchers to use droplet-based microfluidic methods to help explore the behaviour of thousands of individual cells, providing a rapid and easy way to screen for new or cryptic cell types.

The Single Cell RNA-Seq Chip – a commercially available version of the prototype PDMS Drop­seq chip described by Macosko et al¹ – is manufactured from glass, offering excellent optical transparency and ensuring high precision. Combined with a very gentle sample loop method – to minimise bead shearing and contamination due to premature cell lysis – it enables rapid, straightforward preparation of single cell cDNA libraries. The chip offers robust and reproducible processing of 1 ml samples in approximately 12 hours, allowing cells to be individually encapsulated with uniquely barcoded RNA capture beads for high throughput sequencing.

To find out more, please visit http://www.dolomite-microfluidics.com/webshop/microfluidic-chips-droplet-chips-single-cell-rna-seq-chip-c-17_19_126/single-cell-rnaseq-chip-1-100-microetch-depth-fluorophilic-p-419

1.    Macosko, Evan Z. et al (2015). Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell, 161(5): 1202-1214.

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