Poster Report To Describe Workflows For Quantitative Serum Cancer Biomarker Discovery

MONMOUTH JUNCTION, N.J. - Sept. 9, 2015 - PRLog -- MONMOUTH JUNCTION, NJ -- Biotech Support Group has prepared a new report describing a quantitative study of cancer sera using a new product for combining albumin depletion, low abundance enrichment and on-bead digestion. It will be presented as a poster report at the 14th Human Proteome Organization World Congress (HUPO 2015), to be held September 27 – 30, 2015 at the Vancouver Convention Centre, in Vancouver, British Columbia, Canada. The report is entitled:

New Proteomic Workflows Combine Albumin Depletion and On-Bead
Digestion, for Quantitative Cancer Serum Biomarkers

The report describes new workflows that support quantitative serum proteomic investigations and highlight several proteins that were observed to be up and down regulated from lung, breast and early stage pancreatic cancer sera. Proteomic workflows that support serum proteomics can be especially challenging for two reasons: 1) the presence of highly abundant proteins and 2) a particularly proteolytic resistant sample type due to the large post-translational carbohydrate presence.

For quantitative proteomics, having workflows that can greatly reduce one or more highly abundant proteins, along with short, efficient and consistent digestions will be highly desirable. We consider the efficiencies gained by an optimized workflow of albumin depletion, low abundance enrichment, and optimized on-bead digestions, to identify and quantify proteins from sera using isobaric (iTRAQ & TMT) labeling.

“I am very pleased that now with our new AlbuVoid™ product for efficient on-bead methods, digestions of serum samples have been shown to be suitable for quantitative proteomics. Convincingly, several of the proteins we differentially observed have also been cited previously, giving us great confidence that our unique workflow is suitable for cancer biomarker discovery applications.” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of proteomic, metabolomic and genomic sample preparation and enrichment products and services. It’s principal products include: AlbuVoid™ & AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ for nucleic acid isolation. For more information, go to http://www.biotechsupportgroup.com

Contact:
Dr. Swapan Roy & Matthew Kuruc
Biotech Support Group LLC
1 Deer Park Drive, Suite M
Monmouth Junction NJ 08852
732-274-2866 Worldwide
800-935-0628 North America
sales@biotechsupportgroup.com
http://www.biotechsupportgroup.com

Reference:

"New Workflows Combine Albumin Depletion and On-Bead Digestion, for Quantitative Cancer Serum Proteomics"
Haiyan Zheng1; Caifeng Zhao1; Meiqian Qian1; Swapan Roy2; Amenah Soherwardy2; Devjit Roy2; Matt Kuruc2
1Rutgers Center for Integrative Proteomics, Piscataway, NJ;
2Biotech Support Group LLC, Monmouth Junction, NJ Biotech Support Group LLC. United States of America