Detecting DNA DSBs with GeneTex’s γ-H2AX Antibody

July 22, 2015 - PRLog -- Arguably the most ominous form of DNA damage is the DNA double-strand break (DSB), and eukaryotic cells have evolved mechanisms to recognize and repair these lesions to protect their genomic integrity. The phosphorylation of histone variant H2AX at Ser139 (γ-H2AX) is an early cellular response to a DSB that serves to amass chromatin remodeling, cell cycle regulatory, and DSB repair factors to the chromatin region surrounding the break. Antibodies that specifically recognize γ-H2AX have become essential tools for chromatin biology research and are playing increasingly important roles in clinical medicine.

GeneTex is very proud to offer an outstanding mouse monoclonal antibody that detects γ-H2AX. This reagent (Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789)) has been validated for ICC/IF, IHC-P, IP, and WB applications. In addition, histone peptide array analysis (performed by EpiCypher, see above) demonstrates the unparalleled specificity of this antibody for the phospho Ser139 modification.

See: http://www.genetex.com//Histone-H2AXS139ph-phospho-Ser139...