MoBiTec Offers Transzyme Methyltransferase Assays to Eliminate the Need for Assay Development

GÖTTINGEN, Germany - May 20, 2015 - PRLog -- In methyltransferases-related research and drug discovery, the first step in developing epigenetic drugs – identification of specific inhibitors for target enzymes – has been plagued by problems in getting methyltransferase enzymes to function correctly in the HTS assays used to detect their activity.

To eliminate this hurdle, BellBrook Labs and Reaction Biology partnered in 2013 to develop the Transzyme Methyltransferase Assay kits as complete solutions for inhibitor screening and profiling with purified methyltransferases enzymes.

By eliminating the need for sourcing and testing of reagents from different vendors and trouble-shooting to optimize assay performance, the new Transzyme kits should accelerate the pursuit of promising epigenetic therapeutic strategies for cancer and other diseases.

Transzyme Methyltransferase Assay kits include everything you need to detect your target HMT:

• Purified enzymes

• Substrates

• Detection reagents

• A simplified Quick Guide protocol with optimal reaction condition

Even  the amount of enzyme and reaction time required for a great assay signal at low substrate consumption is provided, so scientists don’t have to waste time or reagents on assay development.

Transzyme Methyltransferase Assay kits presently available are:

• DNMT1
• HMT DOT1L
• HMTG9a
• HMT MLL4
• HMT PRMT1
• HMT PRMT3
• HMT SET7/9
• HMT SET8
• HMT SUV39H1
• HMT GLP
• HMT G9a-GLP
• HMT PRMT4 (CARM1)
• HMT PRMT8
• HMT EZH2

DNA Methylation Modulates Alcohol Addiction
Alcoholism is a multifaceted disorder caused by both genetic and environmental influences and epigenetic modifications, including histone acetylation and DNA methylation, have been shown to control alcohol-related behaviors. In a recent study, Estelle Barbier, from Linköping University in Sweden, collaborated with scientists at NIH to begin to understand how DNA methylation contributes to the lasting neuroadaptive changes brought on from a history of alcohol dependence.

The team first investigated global DNA methylation patterns in different regions of the brain associated with addiction and found increased methylation in the medial prefrontal cortex of alcohol-dependent rats. DNA methyltransferase 1 (DNMT1) protein, one of three DNMT isoforms, was overexpressed in the mPFC and specifically in neurons.
(J Neurosci. 2015 Apr 15;35(15):6153-64)

Customers in Europe are welcome to visit www.mobitec.com or contact MoBiTec GmbH for BellBrook Labs product and pricing information. Custom quotes are available for bulk orders.

About Bellbrook Labs

BellBrook Labs is dedicated to providing the pharmaceutical industry with enabling screening tools to accelerate the discovery of more effective therapies. As a small, R&D-driven company, BBL identifies areas where there is a strong need but a significant technical hurdle, and then BBL develops a solution. The company is focused on taking creative approaches to new product development and to continually improving the level of service provided to our customers. BellBrook is undergoing a big change with the launch of the first two iuvo™ products and the Invasion Assay Service in 2010. The company has built its reputation on Transcreener®, a biochemical assay platform, and with iuvo BBL is introducing completely new way to perform cellular assays. www.bellbrooklabs.com

About MoBiTec GmbH

MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.

In parallel to its own product lines, MoBiTec distributes products from international companies. MoBiTec products are distributed worldwide, in Germany from their headquarters in Goettingen, in other countries by distributors.

http://www.mobitec.com