Patent Application Cites AlbuVoid™ For Secretome Sample Preparation & Neuroscience Research

MONMOUTH JUNCTION, N.J. - Feb. 23, 2015 - PRLog -- MONMOUTH JUNCTION, NJ -- Biotech Support Group reports on a recent patent application which supports the utility of their proteomic sample preparation technology on albumin depletion for neuroscience research. The patent application is:

Narain, Niven Rajin, and Paula Patricia Narain. "COMPOSITIONS AND METHODS FOR DIAGNOSIS AND TREATMENT OF PERVASIVE DEVELOPMENTAL DISORDER (http://www.freepatentsonline.com/y2015/0023949.html)." U.S. Patent No. 20,150,023,949. 22 Jan. 2015.

The patent provides albumin sample preparation research application to track modulators of disease processes and experiment data on metabolomics, transcriptomics, single nucleotide polymorphisms using sample preparation and an artificial intelligence based informatics platform.  Serum containing media are reduced using columns, reconstituted with buffers and serum albumin is depleted using Biotech Support Group's AlbuVoid™. Culture medium optimization and subsequent modifications to buffer exchange is performed. Protein extraction requires a lysis buffer with protease inhibitors, vortexing, ultrasonication, centrifugation and clean up of debris. Secretome sample preparation of cultures and conditioned media requires reduction using dithiothreitol (DTT), alkylation using iodoacetamide  and  desalting using acetone precipitation. Quantitation of proteins using Bradford assay is performed and reduction, alkylation, digestion using trypsin is performed. Pooled aliquots of tryptic digests  are labeled using iTRAQ,  and analyzed using liquid chromatography mass spectrometry.

About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of proteomic, metabolomic and genomic sample preparation and enrichment products and services. It’s principal products include: AlbuVoid™ & AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ for nucleic acid isolation. For more information, go to http://www.biotechsupportgroup.com

Contact:
Dr. Swapan Roy & Matthew Kuruc
Biotech Support Group LLC
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Monmouth Junction NJ 08852
732-274-2866 Worldwide
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http://www.biotechsupportgroup.com

Patent - Secretome Sample Preparation

Narain, Niven Rajin, and Paula Patricia Narain. "COMPOSITIONS AND METHODS FOR DIAGNOSIS AND TREATMENT OF PERVASIVE DEVELOPMENTAL DISORDER. (http://www.freepatentsonline.com/y2015/0023949.html)" U.S. Patent No. 20,150,023,949. 22 Jan. 2015.

Narain, Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas. "INTERROGATORY CELL-BASED ASSAYS AND USES THEREOF. (http://www.freepatentsonline.com/y2012/0258874.html)" U.S. Patent No. 20,120,258,874. 11 Oct. 2012.

Suggested References

Kumar, Amit, et al. "Coupling enrichment methods with proteomics for understanding and treating disease." PROTEOMICS-Clinical Applications (2015).

Liu, Guowen, et al. "A Novel and Cost Effective Method of Removing Excess Albumin from Plasma/Serum Samples and Its Impacts on LC-MS/MS Bioanalysis of Therapeutic Proteins." Analytical chemistry 86.16 (2014): 8336-8343.