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Follow on Google News | MoBiTec Launches SpeedBlot (His) – The Fastest Western Blot Stain for His-Tagged Proteins EverInstant detection of poly-histidine tagged proteins on nitrocellulose membrane with SpeedBlot (His) is easy and does not require relevant hands-on time. Even 0.1 pmol detection limits can be achieved with this highly sensitive detection reagent.
By: MoBiTec GmbH Poly-histidine tags are often used for affinity purification of poly-histidine- However, the common protocol for the antibody-based detection of the protein of interest is a laborious and time-consuming undertaking. As time is money and to make life easier MoBiTec introduces SpeedBlot (His), a ready-to-use reagent for the detection of poly-histidine tagged proteins in blot assays: SpeedBlot (His) is equally suitable for Western Blot assays and Dot or Slot Blot assays. The detection reagent is made of an optimized anti-histidine antibody gold nanoparticle conjugate and is reactive against C- and N-terminal tags. The protein of interest is specifically stained in pink directly on the blot. As Easy as Ponceau Red but as Specific as ECL Antibody! Features • Fastest Western ever; results in 30-90 min • Specific staining of His-tagged proteins • Sensitive to sub-picomol amounts of protein • Simple incubation, no buffer exchange, etc. • Economic, no readout equipment required • Visually detectable result • Quantitative and permanent signal • Works with C-/ N-terminal tags The product is readily available. For further details please see: http://www.mobitec.com/ Time is money! (Benjamin Franklin) End
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