Characterization of net charge as well as the overall non-specific self-interactions of biotherapeutics proteins is essential in assessing their aggregation propensity. These colloidal stability measurements are often used in the course of formulation to screen out poorly behaved buffer conditions. Both charge and kD are important parameters to indicate colloidal stability, since charge alone may not provide a complete picture: molecules can have a favorable net charge though be de-stabilized by localized factors such as charge asymmetry and hydrophobic residues. This study showcases the unique ability of the Wyatt Möbius to combine the measurement of charge and kD of monoclonal antibodies in parallel.
Two antibodies were characterized in the study: one was known to be stable under a variety of stress conditions and the second had poor stability. Their measured net charge values indicated that both would exhibit ‘Incipient Instability’
Geofrey Wyatt, President of Wyatt Technology highlighted the benefits of the simultaneous measurement of charge and kD, “The simultaneous, fully automated measurement of both variables in a single experiment, reduces the time and resources required to ensure that formulations are suitable for further development for therapeutic applications. The Möbius is truly unique in its capability to provide reliable, reproducible and non-destructive electrophoretic mobility measurements of fragile biomolecules and we believe that this capability will dramatically improve the level of confidence researchers can have in their assessment of the stability of any given protein.”
Based in Santa Barbara, California, Wyatt Technology is the world’s leading provider of instruments for absolute macromolecular and nanoparticle characterization. With over 40 years’ experience developing multi-angle light scattering detectors, working with customers in the biotechnology, chemical, petrochemical, pharmaceutical, academic and government arenas, Wyatt prides itself on its entrepreneurial spirit, and the uniqueness of its offerings. The Company’s groundbreaking technology and uncompromising levels of customer care make Wyatt the global benchmark in its field.
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The Scott Partnership
The Scott Partnership