Lipid removal from plasma-enzyme-nanoparticle mixture by sample pretreatment using Cleanascite™

MONMOUTH JUNCTION, N.J. - March 30, 2014 - PRLog -- MONMOUTH JUNCTION, N.J. -- Authors Pan et al published an article in the journal Theranostics titled, Anti-Angiogenesis Therapy in the Vx2 Rabbit Cancer Model with a Lipase-cleavable Sn 2 Taxane Phospholipid Prodrug using αvβ3-Targeted Theranostic Nanoparticles’, describing nanoparticle formulations for anti-cancer chemotherapy. Research involved developing and characterizing a Sn 2 lipase-labile prodrug of docetaxel Dxtl-PD. Excessive serum and lipase surrounding taxane prodrug in PFC nanoparticles was optimized using Cleanascite™, a lipid removal reagent. In vitro experiment on anti-angiogenic molecule docetaxel prodrug in perfluorocarbon (PFC) nanoparticles cites Cleanascite™ for lipid removal from plasma-enzyme-nanoparticle mixture. Excessive lipids in samples interferes in high performance liquid chromatography. Subsequent to sample pretreatment by implementing the Cleanascite™ protocol, the mixture is centrifuged and supernatant is analyzed by HPLC. Less matrix effects are observed in high performance liquid chromatography analysis as Cleanascite™ removes lipids and does not bind to DNA, RNA, enzymes and proteins in samples. Next, 2-propranol and water was added to red blood cell aliquots and mixture was centrifuged. The RBC fraction is separated from PFC nanoparticles.

Characteristics of Cleanascite™ Lipid Removal Reagent and Clarification

Helps purify antibodies, recombinant proteins, nucleic acids, proteoglycans

Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates

Clarifies saliva and fecal components

Very low protein binding

Does not bind to DNA, RNA, enzymes and proteins

Leaves glycoproteins, antibodies, nucleic acids, hemoglobin, proteoglycans, nucleic acids, serum components(such as hormones, nutrients, globulins, clotting factors, transport proteins) alone

Extends the life of membrane and chromatographic columns.

Enrichment of delipidated tissue samples

Ideal for delipidation treatments for downstream processing of large-scale therapeutic proteins, enzymes and monoclonal antibodies.

Selectively removes lipids, cell debris, lipoproteins, floating fats, impurities from cohn paste, transgenic milk, egg yolk and biological samples for pretreatment of samples prior to purification.

For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification
http://www.biotechsupportgroup.com/node/73

About Biotech Support Group LLC

Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuSorb™& AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ & ProPrep™ for nucleic acid isolation. For more information, go to http://www.biotechsupportgroup.com

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*For research use only

References

Pan, Dipanjan, et al. "Anti-Angiogenesis Therapy in the Vx2 Rabbit Cancer Model with a Lipase-cleavable Sn 2 Taxane Phospholipid Prodrug using αvβ3-Targeted Theranostic Nanoparticles." Theranostics 2014; 4(6):565-578