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Follow on Google News | First Program Announcement DSO National Labs Singapore Presenting Towards PCR-Free, Visual DNA DeteBIODETECTION TECHNOLOGIES 2014 - the 22nd conference in our Detection Technologies conference series - is an internationally recognized meeting for experts in detection & identification of biological threats and point-of-care analytical methods. This conference will review feedback from the end-users on biodefense and biomedical technologies and explore the cutting-edge in R&D and commercialization efforts in the field, including: • Rapid biosensing, biodetection and analysis • Simultaneous detection of multiple threats and pathogens • Discovery and analysis of biosignatures for the detection of host response • Point- • Point- • Advances in microarray and sequencing technologies • Field- • Non-PCR vs. PCR based detection techniques • Issues of sensitivity/ • Animal and plant pathogens • Bioelectronics • DNA chips, nucleic acid sensors and aptasensors • Enzyme- • Immunosensors • Lab-on- • Microfluidics and immobilisation technology • Natural & synthetic receptors (including MIPs) • Organism- • Proteomics, single-cell analysis and cancer-cell detection • Signal transduction technology (including magneto, piezo, optical and direct electrochemical techniques) FEATURED PRESENTATION: Towards PCR-Free, Visual DNA Detection Mahesh Uttamchandani, PhD, Assistant Professor, DSO National Laboratories, National University of Singapore, Singapore We describe novel methods to detect DNA sequence specifically through color change reactions. To transduce molecular recognition events into visual readouts, we have engineered assays which have exploited split DNAzymes and gold nanoparticles. The G-quadruplex DNAzymes were successfully applied to the detection of Salmonella and Mycobacterium DNA, as well as in genotyping a single base difference from within human genomic DNA samples. This was the first time the approach was demonstrated starting from live genomic DNA samples. An integrated workflow was capable of detecting DNA samples through a color change within just 5-15 min (3). The gold nanoparticle method offered much greater sensitivity, enabling the detection of as little as 608 000 copies of the Salmonella genome, by eye, without the need for PCR amplification. These methods thus enable direct on-site visual detection, by bringing sophisticated DNA analysis capabilities to the point of care setting, without the need for cumbersome equipment or a power source. CONFIRMED PRESENTATIONS: Conductive DNA Real-Time Simultaneous Detection of Multiple Targets Fred Albert, PhD, President, Bridger Technologies, Inc. iTIRF – Cell Phone Based Biosensor for Molecular Diagnostics Alexander N. Asanov, PhD, President and CSO, TIRF Labs, Inc The Photonic Nose: A Versatile and Simple Sensing Tool Leonardo Bonifacio, PhD, Research Scientist, Opalux Inc., Canada A Fully Autonomous Biodetection System for Environmental Surveillance Mark Burton, Northrop Grumman Corporation Bioaerosol Standoff Detection Using Lidar Technology Allowing Cloud Mapping and Spectrometric LIF Classification Sylvie Buteau, PhD, Scientist, Defence Research and Development Canada, Canada Direct Multiplexed Virus Detection John H. Connor, PhD, Professor, Dept of Microbiology, Boston University School of Medicine SoundStream – A Microfluidic- Arlene Doria, PhD, CEO, DEFINEQA Inc. Enabling Point of Care Test Development for the STD Market Joany Jackman, PhD, Investigator, Center for Point of Care Tests for STD, Johns Hopkins University School of Medicine Host-response based biodetection by immunosignatures Stephen Albert Johnston, PhD, Co-Director, Center for Innovations in Medicine, Professor, School of Life Sciences, Biodesign Institute, Arizona State University Tool Box of Engineered Microfluidic Components Shortens Development Time and Reduce Risk Leanna Levine, PhD, CEO, Aline, Inc. Magneto-Hydrodynamic Focusing for Point of Care Applications Christian Reis, Group Lead - Biotechnology Processes, Fraunhofer IPA, Germany Application of a Recombinant Topoisomerase for the Specific Enrichment of Prokaryotic DNA Natalia Sandetskaya, PhD, Fraunhofer Institute for Cell Therapy and Immunology IZI, Germany Treatment Guiding Detection of Burkholderia R. Paul Schaudies, PhD, CEO, GenArraytion, Inc. A Novel Thermostable Viral DNA Polymerase Facilitates Point of Care Molecular Detection of DNA and RNA Targets Hemanth Shenoi, PhD, Lucigen Corp Automatic Online Real-Time Detection of Microcystin- Han-Chang SHI, PhD, Professor, School of Environment, Tsinghua University, PR China New MEMS-based Micro-Coriolis Density Measurement Technology Mike Touzin, Endress+Hauser Flowtec, Switzerland Mid-Infrared QC Lasers for Molecular Recognition Mariano Troccoli, PhD, Director of Product Development, AdTech Optics, Inc. Towards PCR-Free, Visual DNA Detection Mahesh Uttamchandani, PhD, Assistant Professor, DSO National Laboratories, National University of Singapore, Singapore Genomic-Based Approach for Tracking Pathogens Willy Valdivia-Granda, PhD, CEO, Orion Integrated Biosciences Inc. Microcantilever Enabled Biodetection Rick Venedam, PhD, Senior Scientist, National Security Technologies, LLC Point of Care Diagnostic Analysis of Samples in Complex Backgrounds Brad Wright, PhD, Vice President, Bridger Technologies, Inc MEDIA CONTACT: Craig Wohlers Knowledge Foundation 2193 Commonwealth Avenue, Box 398 Boston, MA 02135 Phone: (617) 232 7400 ext 205 Fax: (617) 232 9171 email: cwohlers@knowledgefoundation.com End
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