AnaSpec Introduces SensoLyte® 520 TEV (Tobacco Etch Virus) Protease Assay Kit

FREMONT, Calif. - Jan. 8, 2014 - PRLog -- AnaSpec is pleased to announce the recent release of the SensoLyte® 520 TEV Protease Assay Kit. This kit uses a TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and QXL™ 520 quencher. Proteolytic cleavage of this quenched 5-FAM-QXL™ 520 conjugate yields a brightly green fluorescence, which can be continuously monitored at Ex/Em= 490/520 nm. Increase in fluorescence intensity is directly proportional to the TEV protease quantity/activity.

Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins. It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4 °C, making it the protease of choice for fusion proteins or tags removal (1-3).

For more information, please visit http://www.anaspec.com/products/product.asp?id=57834

References
1.         Fang, L. et al. Prot Expr Pur 51, 102-109 (2006).

2.         Blommel, PG. et al. Prot Expr Pur 60, 1-16 (2007).

3.         Lucast, LJ. et al. Biotechniques 30 (3), 544 (2001).

AnaSpec, EGT Group

AnaSpec, EGT Group is a leading provider of integrated proteomics and genomics solutions^TM for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and Takyon™ qPCR MasterMixes. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery.  AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis.  AnaSpec is certified to ISO 9001:2008.

For more information visit www.anaspec.com