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AnaSpec Introduces SensoLyte® 520 TEV (Tobacco Etch Virus) Protease Assay Kit

SensoLyte® 520 TEV (Tobacco Etch Virus) Protease Assay Kit is used for measuring TEV protease quantity and activity. TEV protease is widely used to remove fusion tags from recombinant proteins.

FREMONT, Calif. - Jan. 8, 2014 - PRLog -- AnaSpec is pleased to announce the recent release of the SensoLyte® 520 TEV Protease Assay Kit. This kit uses a TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and QXL™ 520 quencher. Proteolytic cleavage of this quenched 5-FAM-QXL™ 520 conjugate yields a brightly green fluorescence, which can be continuously monitored at Ex/Em= 490/520 nm. Increase in fluorescence intensity is directly proportional to the TEV protease quantity/activity.

Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins. It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4 °C, making it the protease of choice for fusion proteins or tags removal (1-3).

For more information, please visit http://www.anaspec.com/products/product.asp?id=57834

1.         Fang, L. et al. Prot Expr Pur 51, 102-109 (2006).

2.         Blommel, PG. et al. Prot Expr Pur 60, 1-16 (2007).

3.         Lucast, LJ. et al. Biotechniques 30 (3), 544 (2001).

AnaSpec, EGT Group

AnaSpec, EGT Group is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and Takyon™ qPCR MasterMixes. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery.  AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis.  AnaSpec is certified to ISO 9001:2008.

For more information visit www.anaspec.com

Media Contact
Cecilia Po

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Source:AnaSpec, EGT Group
Location:Fremont - California - United States
Industry:Biotech, Science
Tags:Sensolyte, TEV assay, Tobacco Etch Virus
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