Cryptosporidium RT – PCR Detection Kit is now With AccuVis Bio, Abu Dhabi, UAE

ABU DHABI, UAE - Oct. 17, 2013 - PRLog -- Cryptosporidium is a parasite found in water that causes an infection in mammals termed cryptosporidiosis. It is one of the most common water-bourne diseases and is found world-wide. It affects the intestines of mammals and typically causes an acute short-term infection. The most common symptom is self-limiting diarrhea in healthy individuals, however in immunocompromised individuals the symptoms are particularly severe and often fatal. There is no specific treatment for cryptosporidiosis other than fluid rehydration and management of any pain. Therefore early detection of Cryptosporidium in water is the foremost action to prevent the infection.

AccuVis Bio's Cryptosporidium RT-PCR Detection Kit is a ready-to-use system for the isolation and detection of Cryptosporidium from water samples. First, the kit contains components for the rapid isolation of total RNA from the water samples using spin-column chromatography based on Norgen’s proprietary resin (Nucleic Acid Isolation). Second, the kit contains Cryptosporidium Master Mix to allow for PCR Ampification, as well as a Control Master Mix to allow for the amplification of both an isolation control and a PCR control.  The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed based on real-time PCR using melt curves (Detection). Please see the flowchart to the right.

The Cryptosporidium Master Mix contains reagents and enzymes for the specific amplification of a 218 bp region of the Cryptosporidium genome. In addition, Norgen’s Cryptosporidium RT-PCR Detection Kit contains a second Master Mix, the control 2X RT-PCR Master Mix, which can be used to identify possible PCR inhibition and/or inadequate isolation via a separate RT-PCR reaction with the use of the provided PCR control (PCRC) or Isolation Control (IsoC), respectively.  This kit is designed to allow for the testing of 24 samples. The Cryptosporidium RT- PCR Primer Set and Controls are also available separately for end point RT-PCR detection.