NAMSR News: New approach enables the sorting of cells up to 38 times faster

ARLINGTON, Va. - Oct. 8, 2013 - PRLog -- A team of engineers led by computer scientists at the University of California, San Diego, has developed a new approach that marries computer vision and hardware optimization to sort cells up to 38 times faster than is currently possible. The approach could be used for clinical diagnostics, stem cell characterization and other applications.

The approach improves on a technique known as imaging flow cytometry, which uses a camera mounted on a microscope to capture the morphological features of hundreds to thousands of cells per second while the cells are suspended in a solution moving at approximately 4 meters per second. The technique sorts cells into different categories, for example benign or malignant cells, based on their shape and structure. If these features can be calculated fast enough, the cells can be sorted in real-time.

Other researchers had previously discovered that the physical properties of cells could provide useful information about cell health, but previous techniques had been confined to academic research labs because measuring the cells of interest could take hours or even days. The new approach brings imaging flow cytometry closer to being used in a clinical setting.

The microscope-mounted camera used in imaging flow cytometry operates at 140,000 frames per second. But algorithms currently in use take anywhere from 10 seconds to 0.4 seconds to analyze a single frame, depending on the programming language used - making the technique impractical.

The researchers' new approach speeds processing speeds up to 11.94 milliseconds and 151.7 milliseconds depending on the type of hardware used. For the fastest results, engineers developed a custom hardware solution using a field-gate programmable array, or FPGA, which speeds up the process considerably. The slower results, which are still much faster than what's currently available, were obtained using a graphics processing unit, or GPU.

The researchers' ultimate goal is to analyze the cell properties in real-time, and use that information to sort the cells. To do so, the sorting decision must be made in less than 10 milliseconds.

The computer scientists presented their findings in September at the International Conference on Field Programmable Logic and Applications in Portugal.

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