Multiplex qPCR: IDT Covers How To Get Started

CORALVILLE, Ind. - April 29, 2013 - PRLog -- Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, features a “Core Concepts” article highlighting useful tips for multiplex qPCR users in the recently released April 3.2 issue of the IDT DECODED newsletter. The article provides recommendations and step-by-step considerations from the IDT qPCR experts that will help researchers to make informed decisions about their multiplex qPCR experiments, including: general set-up, assay design, and whether or not multiplex qPCR is the right choice for existing applications.

Multiplex qPCR can be an extremely attractive amplification method, allowing scientists to quickly measure the expression levels of several targets simultaneously, enhancing experimental efficiency. However, successful multiplexing requires attention to experimental design. For example, target amplification can be influenced by numerous factors such as gene expression levels, primer interactions, and competition for reaction reagents. IDT produced these guidelines as a valuable reference for new and experienced users of multiplex qPCR, providing key considerations and technique fundamentals to maximize approach success.

The full piece, along with real scientific application examples and links to the useful IDT online primer design and analysis tools, can be found in the 3.2 issue of DECODED.

For more information please visit www.idtdna.com. Follow us on twitter @idtdna (http://www.twitter.com/idtdna) for real-time updates and insights.

About IDT

Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the world, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; Leuven, Belgium; and Singapore. For more information visit www.idtdna.com.