A simple and quick method for hemoglobin depletion using HemogloBind™

MONMOUTH JUNCTION, N.J. - May 1, 2013 - PRLog -- MONMOUTH JUNCTION, NJ.– Proteomic profiling of erythrocyte proteins to identify novel proteins linked to diseases is an evolving field of clinical proteomics. Cytosolic proteins could contribute to pathology of diseased erythrocytes. Hemoglobin interferes with LC-MS/MS analysis of low abundance cytosolic proteins. Hemoglobin depletion of cytosolic proteins is essential for proteomic sample preparation. Authors Gloria Alvarez-Llamas et al published a chapter in the book Vascular Proteomics titled, Characterization of membrane and cytosolic proteins of erythrocytes, which cites HemogloBind™ from Biotech Support Group for hemoglobin depletion of erythrocyte cells from the cytosolic fraction. A simple method of hemoglobin depletion using HemogloBind™ protocol allows for subsequent downstream proteomic analysis using 2-DE as it reduces major interference of hemoglobin from samples of red blood cells (RBCs) and identifies proteins.  

For detailed protocols and more information click
http://link.springer.com/protocol/10.1007/978-1-62703-405-0_6

Characteristics Of HemogloBind™

Has a high degree of specificity for hemoglobin binding

Applications in blood substitutes, enzyme recovery and analytical interferences

Removes hemoglobin from any species including human, sheep, bovine, goat, etc

Removes hemoglobin from organs, tissues.

Hemoglobin removal from blood, frozen blood, blood spot and from hemolyzed serum.

Hemoglobin removal from red blood cell lysate for proteomics and biomarker drug discovery

For more information on HemogloBind™ click
http://www.biotechsupportgroup.com/node/75

About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, and ProCipitate™ & ProPrep™ for nucleic acid isolation. Currently, Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a proteomics platform used in functional profiling for proteomic analysis and a separations method for generating sub-proteomes used in biomarker and functional proteomic prospecting. For more information, go to: www.biotechsupportgroup.com  

CONTACT:
Dr.Swapan Roy
Biotech Support Group
1 Deer Park Drive, Suite M,
Monmouth Junction, NJ 08852, USA
732-274-2866
sales@biotechsupportgroup.com

HemogloBind™ References

Alvarez-Llamas, Gloria, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, Maria Posada-Ayala, and Fernando Vivanco. "Characterization of Membrane and Cytosolic Proteins of Erythrocytes." In Vascular Proteomics, pp. 71-80. Humana Press, 2013

Walpurgis, Katja, et al. "Validated hemoglobin‐depletion approach for red blood cell lysate proteome analysis by means of 2D PAGE and Orbitrap MS." Electrophoresis 33.16 (2012): 2537-2545.

Suggested References

Goodman, Steven R., et al. "The human red blood cell proteome and interactome." Experimental Biology and Medicine 232.11 (2007): 1391-1408