The FRET peptide in the SensoLyte® 520 kit employs the QXL™ 520/HiLyte Fluor™ 488 pair for optimal quenching of the intact substrate. Hydrolysis of the FRET substrate by active Cathepsin L results in increase of HiLyte Fluor™ 488 fluorescence. Additionally, the fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, the pH at which Cathepsin L activity is optimal. This homogeneous assay can be used for the detection of Cathepsin L activity in biological samples or in purified enzyme preparations. The SensoLyte® Rh110 Cathepsin L Activity Assay Kit provides a convenient assay for screening Cathepsin L inhibitors or assay of enzyme activity using Rh110 (rhodamine 110). Upon cleavage by Cathepsin L, this substrate generates Rh110 emitting bright green fluorescence.
Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases (1). It is involved in the promotion of tumor cell invasion (2), antigen processing and turnover of intracellular and secreted proteins (3). It also plays a major role in growth regulation, bone resorption and metastasis (4, 5).
1. Barrett, A.J. et al. Meth Enzymol 80, 535 (1981).
2. Gottesman, M.M. et al. Biochem 20, 1659 (1981).
3. Ishidoh, K. et al. Biochem Biophys Res Commun 217, 624 (1995).
4. Kane, S.E. et al. Semin Cancer Biol 1, 127 (1990).
5. Kirschke, H. et al. Eur J Cancer 36, 787 (2000).
For more information, please visit http://www.anaspec.com/
AnaSpec, EGT Group
AnaSpec, EGT Group is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and qPCR MasterMixes. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.
For more information visit www.anaspec.com