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BioVision Introduces Two New Assay Kits: Creatine Kinase Activity & Xanthine/Hypoxanthine Assay Kit

BioVision is thrilled to introduce two brand new assay kits: Creatine Kinase Activity Assay Kit, a highly sensitive kit for measuring Creatine Kinase Activity and Xanthine/Hypoxanthine Assay Kit, which is a metabolic/cell signaling assay kit.

PRLog - Dec. 28, 2012 - MILPITAS, Calif. -- Creatine Kinase (CK) Activity Colorimetric Assay Kit [Cat # K777-100 (100 Assays)]: Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP:creatine N-phosphotransferase is a common cellular enzyme (EC It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine. CK is widely expressed in various tissues and cell types, with highest activity in striated muscles, heart tissue and brain. CK consists of two subunits: M (muscle) and B (brain), and has three isoenzymes: CK-MM (skeleton muscle), CK-MB (cardiac muscle), and CK-BB (brain). Increased CK level is associated with many diseases such as myocardial infarction, muscular dystrophy, pulmonary infarction and brain tumors. Accurate measurement of CK is crucial for early diagnosis, prediction and therapeutic strategy. In BioVision’s Creatine Kinase Activity Colorimetric Assay kit, creatine kinase converts creatine into phosphocreatine and ADP. The generated phoshocreatine and ADP reacts with CK Enzyme Mix to form an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm.

Key Features and Benefits of the Kit
- Simple Procedure; takes ~ 30-40 minutes
- Rapid, Sensitive & High-Throughput Adaptable
- Versatile: can be used with animal tissues, adherent or suspension cells, serum, plasma, etc.
- Can detect Creatine Kinase activity less than 1 mU
- Colorimetric quantification (OD = 450 nm)
- Kit provides ample reagents to perform 100 assays in a 96-well plate format

For more information, please visit, http://www.biovision.com/creatine-kinase-activity-colorim...

Xanthine/Hypoxanthine Assay Kit [Cat # K685-100 (100 Assays)]: Xanthine, a catabolic product of purine metabolism, is present in body fluids, muscle tissue and certain plants. Structurally like caffeine, Xanthine has a stimulant effect and is used clinically to treat the congestive diseases such as asthma and chronic obstructive pulmonary disease. Xanthine is metabolized into uric acid and superoxide by Xanthine oxidase. Xanthine oxidase deficiency causes the rare genetic disorder-Xanthinuria, and leads to Xanthine accumulation in urine and blood, which ultimately progresses to renal failure. Recent studies show that Xanthine levels are elevated following ischemic injury, thus Xanthine can serve as a useful marker for tissue hypoxia. Early detection of Xanthine alteration in biological fluids is crucial for metabolic studies and for diagnostic and therapeutic monitoring. In BioVision’s Xanthine/Hypoxanthine Assay kit, Xanthine/Hypoxanthine is specifically oxidized by the Xanthine Enzyme Mix to form an intermediate, which reacts with Developer & Probe to form a product that can be measured colorimetrically (λ = 570 nm) or fluorometrically (Ex/Em = 535/587 nm).

Key Features and Benefits of the Kit
- Simple, Reliable and Highly Sensitive
- Rapid, Convenient and High-Throughput Adaptable
- Colorimetric (OD  = 570 nm) or Fluorometric (Ex/Em = 535/587 nm) quantification
- Can detect Xanthine/Hypoxanthine level as low as 0.4 µM in various biological samples
- Versatile: can be used with animal tissues, adherent or suspension cells, body fluids: serum, plasma, urine etc. & cell & tissue culture supernatant
- Kit provides ample reagents to perform 100 assays in a 96 well plate format

For more information, please visit, http://www.biovision.com/xanthine-hypoxanthine-assay-kit-...

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Source:BioVision, Inc.
Location:Milpitas - California - United States
Tags:creatine kinase assay, CK assay kit, xanthine/hypoxanthine kit, CPK activity assay
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