Albumin depletion using Albusorb™ from serum samples to research glycoprotein Apolipoprotein C-III

PRLog (Press Release) - Nov. 11, 2012 - MONMOUTH JUNCTION, NJ.– Apolipoprotein C-III is an important protein component of triglyceride rich lipoproteins and HDL. The glycoprotein Apolipoprotein C-III (apoCIII) inhibits lipolysis and it’s expression is documented to play a vital role in the development of hypertriglyceridemia when increased. The aim of this study was to determine apoCIII’s increased levels in serum in T1D patients and that it affects the function and survival of pancreatic β cells in vitro. Authors Holmberg et al published an article titled, “Lowering apolipoprotein CIII delays onset of type 1 diabetes” in the journal Proceedings of the National Academy of Sciences in which Albusorb™ from Biotech Support Group is used to remove albumin from rat serum samples. Scientists setup experiments implementing the animal model diabetes-prone BB rat (DPBB) to ascertain if apoCIII increases contributed to calcium increase and B-cell death in vivo. To evaluate the levels of apoCIII in positive, negative, and control sera, scientists used Albusorb™ (Biotech Support Group) to remove albumin from serum samples. For detailed experimental research studies click http://www.pnas.org/content/early/2011/06/08/1019553108.a...

Albusorb™ is an albumin depletion reagent supplied as a kit with necessary buffers. AlbuSorb™ comes from a silica-based, separation platform utilizing a new combination of surface microenvironments substituted with low molecular substrates that feature drug-binding motifs. Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination.

Characteristics of Albusorb™

Removes >90% albumin from 30 mg albumin/ml sample
Affinity-type equivalence, virtually no cross-reactivity with other proteins
Bind and elute procedure - simply weigh powder, condition the sample, centrifuge and/or filter, and recover the albumin depleted serum
Economical new surface technology, not based on affinity chromatography
Mild conditions maintain tertiary structure of proteins and simple transfer to secondary analysis
The albumin depleted filtrate retains the enzymatic and biological activity
Removes albumin from samples such as serum, plasma and from species including human, mouse, sheep, bovine, goat, rat, and calf
The flow through fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.

For more information about AlbuSorb™, click:
AlbuSorb™ Albumin Depletion Kit
http://www.biotechsupportgroup.com/node/64

About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include:  Albusorb™ for albumin depletion, AlbuVoid™ for albumin depletion plus low abundance serum protein enrichment, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, and ProCipitate™ & ProPrep™ for nucleic acid isolation. Biotech Support Group is the leading developer of sample preparation products for separating and purifying hemoglobin from blood samples. HemoVoid™ is a hemoglobin depletion reagent kit from red blood cells and HemogloBind™ is a hemoglobin capture reagent from hemolyzed serum. Currently, Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a proteomics platform used in functional profiling for proteomic analysis and a separations method for generating sub-proteomes used in biomarker and functional proteomic prospecting. For more information, go to: http://www.biotechsupportgroup.com/  

CONTACT:
Ravish Amin
Biotech Support Group
1 Deer Park Drive, Suite M,
Monmouth Junction, NJ 08852, USA          
732-274-2866
sales@biotechsupportgroup.com

AlbuSorb™ References

Rat Serum

Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.
http://www.pnas.org/content/early/2011/06/08/1019553108.abstract?elq=06e83b82098d4e9798d154c3ad738c83

Cerebrospinal Fluid

Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270
http://www.springerlink.com/content/j4u06x2k73u11476/#section=1013451&page=1

Serum

Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex.Journal of Biological Chemistry.2012;287(11):8092-100
http://www.ncbi.nlm.nih.gov/pubmed/22267731

Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.
http://www.pnas.org/content/early/2011/06/08/1019553108.abstract?elq=06e83b82098d4e9798d154c3ad738c83

Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats  Nutrition and Cancer.2011;63(2):227-33
http://www.researchgate.net/publication/49814854_Effects_of_Nobiletin_on_PhIP-Induced_Prostate_and_Colon_Carcinogenesis_in_F344_Rats

Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis
http://publications.ki.se/jspui/handle/10616/39507

Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Analytical Chemistry.2009;81(21):8715-23
http://www.ncbi.nlm.nih.gov/pubmed/19788250