Rainin Instrument, a METTLER TOLEDO subsidiary, has published a new white paper entitled "High Throughput FPLC with Packed Resin Tip Technology."
Using conventional spin columns, protein purification requires a relatively large amount of resin for acceptable performance. However, experiments detailed in the white paper indicate that the higher resin volumes required in spin columns are not the key to optimizing workflow.
PureSpeed's bidirectional flow was shown to load PureSpeed's relatively small resin bed with the most protein possible, providing superior final concentrations and purity when compared to the spin column procedure. Protein capture was shown to be more repeatable as well, even at significantly reduced elution volumes.
The improved concentrations that PureSpeed enables help provide samples robust enough for assaying or downstream processing. Generally, this saves time by eliminating the need to concentrate recovered protein in a separate step.
The paper also offers guidelines for using PureSpeed tip, body and bed sizes to gain maximum protein/antibody processing.
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METTLER TOLEDO specializes in the area of precision instruments for professional use. METTLER TOLEDO laboratory instruments are used in research, scientific, drug discovery, and quality control labs, amongst many others in the pharmaceutical, chemical, food and cosmetics industries. METTLER TOLEDO comprehensive industrial solutions cover the various steps in a host of manufacturing processes at many of the same customers that laboratory serves. Solutions range from receiving raw materials through various manufacturing processes, in-line process control and end-of-line packaging control, to logistics and shipping. Increasingly, these solutions are fully integrated into the customer's IT environment, helping automate their workflows. In food retail, our offering for fresh food management ranges from receiving and pre-packaging, to in-store solutions for self-service departments, deli counters, and checkout terminals.