BioVision Introduces Two New Assay Kits: InSitu HDAC Activity Assay and Total Carbohydrate Assay Kit

PRLog (Press Release) - Oct. 31, 2012 - InSitu HDAC Activity Fluorometric Assay Kit [Cat # K339-100 (100 Assays)]: Histone acetylases (HAT’s) and Histone deacetylases (HDAC’s) are associated with regulation of gene expression. In general, increased levels of histone acetylation are associated with increased transcriptional activity, whereas decreased levels of acetylation are associated with repression of gene expression. HDAC’s are localized in both the cytosol and nucleus and some shuttle between the nucleus and cytosol. Increased HDAC expression has been observed in various cancers. BioVision’s InSitu HDAC Activity Fluorometric Assay Kit provides a direct, fast, fluorescence-based method to measure the InSitu HDAC activity. The procedure requires two easy steps, all performed in the same cell culture plate. First, the cell permeable HDAC Substrate, which comprises an acetylated lysine side chain, is incubated with cells grown in a 96-well plate. Inside the cells, HDAC deacetylates the substrate. The second step involves lysing the cells and treating with the Developer that produces a fluorophore from the Deacetylated HDAC Substrate. The generated fluorescence can be quantified at Ex/Em = 368/442 nm. The assay is well suited for high throughput screening applications.

Key Features and Benefits of the Kit
● Super-fast Protocol and non-radioactive
● Simple; just requires two easy steps    
● Suitable for High-Throughput  
● Applicable to both adherent as well as suspension cells
● Designed to perform whole experiment in the same cell culture plate
● Fluorometric quantification (Ex/Em = 368/442 nm)
● Kit provides ample reagents to perform 100 assays in a 96-well plate format

For more information, please visit, http://www.biovision.com/insitu-hdac-activity-fluorometri...

Total Carbohydrate Assay Kit [Cat # K645-100 (100 Assays)]: Carbohydrates play important structural as well as chemical roles in all living systems. Detection of total carbohydrates, therefore, has wide applications. BioVision’s Total Carbohydrate Assay is a simple, sensitive and robust method of detecting virtually all carbohydrates. The assay is based on the phenol-sulfuric acid method. In Total Carbohydrate Assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural, which react with the Developer to form a chromogen that can be quantified by measuring the absorbance at 490 nm. The Total Carbohydrate Assay can detect most forms of carbohydrates, including simple and complex saccharides, glycans, glycoproteins and glycolipids.

Key Features and Benefits of the Kit
● Simple and convenient
● Rapid (~25 min.) and reliable
● Colorimetric quantification (OD = 490 nm)
● Versatile: Can be used with animal and plant tissues, adherent or suspension cells as well as food products, fruit juices & other beverages.
● Kit provides ample reagents to perform 100 assays in a 96-well plate format

For more information, please visit, http://www.biovision.com/total-carbohydrate-assay-kit-579...