BioVision Introduces Two New Assay Kits: SIRT2 Inhibitor Screening Assay Kit & Hexokinase Assay Kit

PRLog (Press Release) - Aug. 30, 2012 - SIRT2 Inhibitor Screening Assay Kit (Cat # K322-100): Sirtuin or Sir2 proteins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins have been implicated in influencing aging and regulating transcription, apoptosis and stress resistance, as well as energy efficiency and alertness during low-calorie situations. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction couples lysine deacetylation to NAD hydrolysis. This hydrolysis yields O-acetyl-ADP-ribose, the deacetylated substrate and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. In BioVision’s Sirtuin inhibitor screening Kit, Sirtuin deacetylates the substrate and then the developer cleaves the deacetylated substrate to release the fluorescent group, which can be detected fluorometrically at Ex/Em = 395/541 nm. In the presence of SIRT inhibitor, the deacetylation will be impeded which prevents the cleavage of the substrate to release the fluorescent group.

Key features and benefits of the kit:
•   Simple and convenient
•   Rapid, reliable and sensitive
•   Suitable for high-throughput
•   Effective for screening SIRT2 inhibitors
•   Comes with Inhibitor control (Nicotinamide)
•   Fluorometric quantification (Ex/Em = 395/541 nm)
•   Kit provides ample reagents to perform 100 assays in a 96 well plate format

For more information, please visit, http://www.biovision.com/sirt2-inhibitor-screening-assay-...

Hexokinase Colorimetric Assay Kit (Cat # K789-100): Hexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km. Hexokinase deficiency leads to severe human diseases such as X-linked muscular dystrophy and a rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis. Early detection of abnormal hexokinase activity is crucial for diagnosis, prediction and treatment of the disease. In BioVision’s Hexokinase Assay kit, glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.

Key features and benefits of the kit:
•   Simple, convenient and non-radioactive
•   Rapid, reliable and highly sensitive
•   Colorimetric quantification (OD = 450 nm)
•   Can detect as low as 0.1 mU per well (based on our unit definition)
•   Versatile: can be used with serum as well as animal tissues and cells
•   Kit provides ample reagents to perform 100 assays in a 96 well plate format

For more information, please visit, http://www.biovision.com/hexokinase-colorimetric-assay-ki...