BioVision Introduces Industry’s Simplest and Fastest Glucose Uptake Assay Kits

PRLog (Press Release) - Jul. 18, 2012 - Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. Like glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells.

Key Features and benefits of the Kits
• Simple, convenient and non-radioactive
• Super-fast Protocol
• Highly Sensitive, detects glucose uptake as low as 10 pmol/well
• High-Throughput Adaptable
• Can be used for a variety of cell types
• Fluorometric (Ex/Em = 535/587) or Colorimetric (λ = 412 nm) quantification
• Kit provides ample reagents to perform 100 assays in a 96 well plate format
• Applicable to various fields like diabetes and obesity research, insulin signaling,  screening diabetes related drugs etc.

Glucose Uptake Fluorometric Assay Kit (Cat # K666-100): In BioVision’s glucose uptake fluorometric assay kit, the accumulated 2-DG6P is enzymatically oxidized and coupled to BioVision’s PicoProbe^TM, which generates fluorescence in the presence of NADPH. The Kit detects glucose uptake as low as 50 pmol/well.

For more information, please visit, http://www.biovision.com/glucose-uptake-fluorometric-assa...

Glucose Uptake Colorimetric Assay Kit (Cat # K676-100): In BioVision’s glucose uptake colorimetric assay kit, the 2-DG6P is oxidized to generate NADPH, which can be determined by an enzymatic recycling amplification reaction. The Kit detects glucose uptake as low as 10 pmol/well.

For more information, please visit, http://www.biovision.com/glucose-uptake-colorimetric-assa...