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Follow on Google News | Cleanascite™ for lipid removal and identification of biomarkers from fish bile samples.Cleanascite™ for lipid removal and identification of biomarkers for environmental contamination from fish bile samples containing metalloproteinases
By: Biotech Support Group MMPs are protease enzymes which require a metal (zinc or cobalt) for catalytic activity. MMPs function in wound repair, tissue development and embryogenesis by destroying extracellular matrix of normal physiological processes. Examples of MMPs are gelatinases, collagenases, stromelysins and similar proteinases. Published papers by authors Shapiro have shown the connection between overexpression of a specific MMP, either by tumor cells or the surrounding stroma, as being pro-tumorigenic. Moreover MMPs are recruited by tumor cells for reconstructing normal tissue and promote neovascularization, tumor spread and growth. Synthetic MMP inhibitors are being developed for slowing tumor growth and for anti-neoplastic therapy. Gallbladder bile from 2 fish species, mullet (Mugil liza) and tilapias (Tilapia rendalli), contained substantial matrix metalloproteinases (MMPs). Extensive purification studies were conducted in order to obtain workable samples for SDS-PAGE and zymography analysis. Cleanascite™ Fish is ideal for assessing water-borne or sediment-deposited toxins, provides knowledge of environmental contamination due to toxicants and therefore comparing fish proteomes to analyze proteome expression under complex field situations could provide knowledge of gene products, metabolites, or proteins for further studies. (Albertsson et al. 2007). Metalloproteins in fish could be used as biomarkers of environmental exposure for fish and have utility as biomarkers for environmental contamination (López-Barea and Gómez-Ariza 2006 ). Quantifying total metal levels in fish organs, and directly relating them to environmental contamination provides information about toxic exposure levels (Moldovan et al. 2004 ).Total metal levels in fish are influenced by the amount of metals present in the form of metal-bound proteins and/or on-site contamination. About Biotech Support Group LLC Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuVoid™ CONTACT: Ravish Amin Biotech Support Group 1 Deer Park Drive, Suite M, Monmouth Junction, NJ 08852, USA 732-274-2866 sales@biotechsupportgroup.com References for Cleanascite™ Hauser-Davis RA, Lima AA, Ziolli RL, Campos RC.First-time report of metalloproteinases in fish bile and their potential as bioindicators regarding environmental contamination. Aquatic Toxicology.2012; Farina A, Dumonceau JM, Frossard JL. Proteomic Analysis of Human Bile from Malignant Biliary Stenosis Induced by Pancreatic Cancer Journal of Proteome Research.2009; Guerrier L, Claverol S, Finzi L et al. Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins. Journal of Chromatography.2007; Chen Bo, Zheng Jian-wei, Wang Jian-ming, et al. Establishment and preliminary analysis of a 2-D human biliary map Chinese Journal of Hepatobiliary Surgery.2007 Chen B, Dong JQ, Chen YJ et al Two-dimensional electrophoresis for comparative proteomic analysis of human bile. Hepatobiliary & pancreatic diseases international.2007 Aug;6(4):402- Guerrier L, Claverol S, Finzi L et al Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins.Journal of Chromatography A.2007;1176( Kristiansen TZ, Bunkenborg J, Gronborg M et al A Proteomic Analysis of Human Bile Molecular and Cellular Proteomics.2004; End
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