Fitzco ProPRIME Collection Cards

Oct. 31, 2011 - PRLog -- Benefits of the Fitzco ProPRIME Collection Card
 Easy Sample Collection
 Long term protein stabilization of primary structures
 Long term Nucleic Acids preservation
 Efficient - Ready for processing in minutes
 Storage - Ambient or Frozen
 Available in Indicating - for collection and processing ease with transparent specimens
 Kills pathogens

ProPRIME utilizes a gentle cell lysis upon contact unlike existing paper matrix product(s) utilizing harsh extraction techniques and methods. ProPRIME solves the problem of protein denaturing during the lysis phase of a biological sample. Cell lysis is the disruption of the cell walls to release the biological components of the cell. These include DNA, RNA, organalles, and lipids from the cell.

Once the cell has lysed and spilled out its cellular contents, the cell has died. Cell lysis can occur naturally, in most cases through the drying process. It can also occur chemically and physically. Usually when a sample dries the cell and its components lyse onto a matrix or substrate. Cell lysis fractures the cell and releases its components entrapping the cellular contents within the matrix. As that occurs, the protein can denature or degrade and become essentially useless. Other commercially available matrix lysing products that instantly lyse cells are harsh and do not provide a suitable environment for the protein, hence denatures protein structures.

Primary protein structures are preserved. Proteins are the target with other cellular components (i.e. nucleic acids) trapped in the treated FP705™ paper as well. ProPRIME also kills pathogens.

ProPRIME is proven to work with many current protocols. Applications with current paper matrix collection products using existing DNA processing techniques and protocols work with a slight modification or additional wash step. PRO is also amendable to automation for DNA analysis. Liquid handling systems can be utilized for protein or DNA extractions that include the silica-based extraction chemistries.

Benefits of the Fitzco PRO Collection Card

 Easy Sample Collection
 Long term protein stabilization of primary, secondary and tertiary structures
 Long term Nucleic Acid preservation
 Efficient - Ready for processing in minutes
 Storage - Ambient or Frozen
 Available in Indicating - for collection and processing ease with transparent specimens

PRO utilizes a gentle cell lysis upon contact unlike existing paper matrix product(s) utilizing harsh extraction techniques and methods. PRO solves the problem of protein denaturing during the lysis phase of a biological sample. Cell lysis is the disruption of the cell walls to release the biological components of the cell. These include DNA, RNA, organalles, and lipids from the cell.

Once the cell has lysed and spilled out its cellular contents, the cell has died. Cell lysis can occur naturally, in most cases through the drying process. It can also occur chemically and physically. Usually when a sample dries the cell and its components lyse onto a matrix or substrate. Cell lysis fractures the cell and releases its components entrapping the cellular contents within the matrix. As that occurs, the protein can denature or degrade and become essentially useless. Other commercially available matrix lysing products that instantly lyse cells are harsh and do not provide a suitable environment for the protein, hence denatures protein structures.

Primary, secondary, and tertiary protein structures are preserved. Proteins are the target with other cellular components (i.e. nucleic acids) trapped in the treated FP705™ paper as well.

PRO is proven to work with many current protocols. Applications with current paper matrix collection products using existing DNA processing techniques and protocols work with a slight modification or additional wash step. PRO is also amendable to automation for DNA analysis. Liquid handling systems can be utilized for protein or DNA extractions that include the silica-based extraction.

For further details on our range of cards please visit http://www.fitzco.co.uk

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Since 1991, Fitzco has manufactured samples collection products for organisations all over the world. Fitzco are the original co-founder of the FTA cards and Omni Swab. Our products serve the requirements of forensic and diagnostic fields the world over.