GenScript Upgrades OptimumGene™ Gene Design System

PR Log (Press Release) – Nov 03, 2008 – PISCATAWAY, N.J.,  –International life science contract research organization (CRO) GenScript Corporation continuously integrates new techniques and technology into its service platforms.  GenScript's OptimumGene™ gene design platform has reached new levels of efficiency and precision in gene synthesis and expression.  
GenScript's OptimumGene™ system's primary purpose is to redesign genes of interest to improve protein expression in research settings.  Principally, it can be very difficult to express a gene of interest in an organism not its own.  For example, a laboratory may need to express a human gene in E. coli or cultured mouse cells.  Many of GenScript's customers have found that altering the codon bias—changing the gene so that it codes for the exact same amino acids in a way better suited to the expression system—can make research quicker, easier, and less expensive.  In addition, OptimumGene™ mitigates the effects of problematic codons, and other problems.

The OptimumGene™ design platform employs a unique algorithm and proprietary codon usage table. The algorithm incorporates factors involved in gene transcription efficacy, translation efficiency, and refolding, such as GC content, CpG dinucleotide content, cryptic splicing sites, negative CpG islands, SD sequence, TATA box, terminal signal, codon usage bias, mRNA secondary structure, premature PolyA sites, internal chi sites and ribosomal binding sites, RNA instability motif, inhibition sites, stable free energy of mRNA, codon-context, the interactions of codon and anti-codons, and RNA secondary structures. Each of these can be adjusted to the customer's specific needs.

Additional restriction enzyme cutting sites can be added for later manipulation of the construct. In addition, the proteins expressed can be designed to incorporate epitope tag sequences (e.g., HIS or GST tag) or protease cutting sites for one-step purification. Libraries of variant forms of mutant genes can also be constructed for functional study and screening. Using this platform, viral genes can be expressed in mammalian cell lines, during which process the regulatory element in mammalian system is disrupted enabling the viral protein production to increase, and in some cases up to 28%. This may be of particular advantage to DNA vaccine development in that it promotes the immunogenicity of the target.  

GenScript Corporation is a reliable outsourcing partner, providing a wide array of services to augment all stages of the drug discovery and biological reagent manufacturing processes, including high-quality gene synthesis and cloning, custom protein expression and purification, custom peptide synthesis and antibody development, and custom stable cell line development.  GenScript also offers a selection of biological reagents to life science researchers in both industrial and academic settings. GenScript Corporation is headquartered in Piscataway, New Jersey.

http://www.genscript.com/gene_design.html
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120 Centennial Ave.
Piscataway,
NJ 08854
USA

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