The structure of each member of the SOCS family consists of a central Src homology 2 (SH2) domain and a SOCS box motif of 40 amino acids on the C-terminus (2,3). The SOCS proteins form part of a classical negative feedback loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism (4). Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways.
AnaSpec has introduced a suite of SOCS antibodies that have been validated in western blots using cell or tissue lysates. These polyclonal antibodies, raised in rabbits and affinity purified, come in a new economical 50 µg format.
Product Size Catalog # Approx MW (kDa) Lysate used in WB
Anti-SOCS-1 50 µg 28132 24 HEK293 (cat# 29520)
Anti-SOCS-2 50 µg 28133 26 Mouse liver (cat# 29509)
Anti-SOCS-3 50 µg 28134 27 MCF-7 (cat# 29519)
Anti-SOCS-4 50 µg 28135 55 Mouse brain (cat# 29507)
Anti-SOCS-5 50 µg 28136 61 A431 (cat# 29514)
For more information, visit www.anaspec.com
AnaSpec, Inc., located in San Jose, CA, is a leading provider of integrated proteomics solutions to pharmaceutical, biotech, and academic research institutions throughout the world. With a vision for innovation through synergy, AnaSpec focuses on three core technologies:
References:
1. Krebs, DL and DJ. Hilton. J. Cell Sci. 113, 2813 (2000).
2. Starr, R. et al. Nature 387, 917 (1997).
3. Hilton, DJ. et al. Proc. Natl. Acad. Sci USA 95, 114 (1998).
4. Yoshimura, A. Cytokine Growth Factor Rev. 9, 197 (1998).
