However, increasing demand for accelerated drug discovery and automated inhibitor screening has generated a need for a continuous, homogeneous assay with even greater sensitivity. With the introduction of the ultra-sensitve EnzoLyteTM 520 Renin Assay Kit, AnaSpec now offers a renin assay kit that is 40x more sensitive than comparable kits currently in the market.
Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels, leading to increased blood pressure. Angiotensin II also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. Since an overactive renin-angiotensin system leads to hypertension, renin is an attractive target for the treatment of this disease.
While existing renin assay kits offer sub-nanomolar potency and selectivity, AnaSpec’s EnzoLyteTM 520 Renin Assay Kit offers picomolar sensitivity – the highest sensitivity available for commercial renin assay kits.
AnaSpec also offers a shorter emission wavelength kit, EnzoLyte™ 390 Renin Assay Kit -a cost-effective option that offers greater sensitivity than EDANS/DABCYL-
EnzoLyte™ 520 Renin Assay Kit
EnzoLyte™ 390 Renin Assay Kit
EnzoLyteTM 520 Renin Assay Kit
FRET pair: 5-FAM/QXLTM520
Ex/Em (nm): 490/520
Min. Detectable Renin Conc. (pM): 2.5
Interference from Autofluorescence:
Sensitivity:
EnzoLyteTM 390 Renin Assay Kit
FRET pair: Mc-Ala/Dnp
Ex/Em (nm): 330/390
Min. Detectable Renin Conc. (pM): 25
Interference from Autofluorescence:
Sensitivity:
Company B
FRET pair:EDANS/DABCYL
Ex/Em (nm): 335/495
Min. Detectable Renin Conc. (pM):100
Interference from Autofluorescence:
Sensitivity:
For more information, visit www.anaspec.com
AnaSpec, Inc., located in San Jose, CA, is a leading provider of integrated proteomics solutions to pharmaceutical, biotech, and academic research institutions throughout the world. With a vision for innovation through synergy, AnaSpec focuses on three core technologies:
